Understanding Enzymes

Function, Design, Engineering, and Analysis

Author: Allan Svendsen

Publisher: CRC Press

ISBN: 9814669334

Category: Mathematics

Page: 700

View: 2558


Understanding Enzymes: Function, Design, Engineering, and Analysis focuses on the understanding of enzyme function and optimization gained in the past decade, past enzyme function analysis, enzyme engineering, and growing insights from the simulation work and nanotechnology measurement of enzymes in action in vitro or in silico. The book also presents new insights into the mechanistic function and understanding of enzyme reactions, as well as touching upon structural characteristics, including X-ray and nuclear magnetic resonance (NMR) structural methods. A major focus of the book is enzyme molecules’ dependency on dynamic and biophysical environmental impacts on their function in ensembles as well as single molecules. A wide range of readers, including academics, professionals, PhD and master’s students, industry experts, and chemists, will immensely benefit from this exclusive book.

Understanding Insulin Action

Principles and Molecular Mechanisms

Author: J. Espinal

Publisher: Springer Science & Business Media

ISBN: 1461314755

Category: Science

Page: 129

View: 1865


Every year between three and four hundred papers are published on the topic of insulin action. This extraordinary publication rate prevents any author from includ ing an exhaustive bibliography in any review or book. Perhaps due to this there is no single text that attempts to cover the effects and the mechanism of action of insulin. This book is such an attempt. I intend to present a review of the physiological effects of insulin, the pathology of defects in the action of insulin, and the current views on the mechanism of action of this hormone. I make no apology for the fact that the bibliography will not be extensive and that the amount of experimental detail and data discussed will be kept to a relevant minimum. This book is not intended for the expert in the field, but for the second- or third-year undergraduate and graduate student of medicine, biochemistry, physiology or related disciplines, and will be valuable as a reference source for research workers. The book is presented as a guide, a summary of the ideas and facts; it will present a reader with a foretaste of a fascinating and ever-changing field. I have attempted to be up-to-date with published research work. Any significant contributions to the field not included in the first draft have been added as footnotes. I assume a basic knowledge of the metabolic pathways of carbohydrates, fats and proteins.

e-O-Level Science Biology Learning Through Diagrams

Author: S.H. Chan

Publisher: Singapore Asia Publishers Pte Ltd

ISBN: 9814387355

Category: Education

Page: N.A

View: 9219


You will find this book interesting: Biology concepts presented in a diagrammatic form. Specially written to ease learning and to stimulate interest in Biology, this book will help students in acquiring and reinforcing Biology concepts, and especially the difficult ones, more easily and effectively. This book makes learning easier through the following features: Learning Outcomes - Learning outcomes on the header point out the concepts that you should focus on in the process of learning. Important Concepts and Key Terms - The important concepts and key terms are presented clearly in simple language. Further explanations linked to the diagrams help you better understand the concepts. Interesting Visuals - Visual aids such as concept maps, flow charts and annotated diagrams are integrated to make the concepts easier to understand and remember. Real-life Examples - These examples show real-life application of concepts and explain the inquiries on the phenomena that happen in our everyday lives. Worked Examples - Step-by-step worked examples help to reinforce your skills in solving problems. Instant Facts - These are extra information that can help you acquire a more in-depth understanding of the topic under discussion. This book complements the school curriculum and will certainly help in your preparation for the examinations.

Exploring Biology in the Laboratory: Core Concepts

Author: Murray P. Pendarvis,John L. Crawley

Publisher: Morton Publishing Company

ISBN: 161731899X

Category: Science

Page: 640

View: 6237


Exploring Biology in the Laboratory: Core Concepts is a comprehensive manual appropriate for introductory biology lab courses. This edition is designed for courses populated by nonmajors or for majors courses where abbreviated coverage is desired. Based on the two-semester version of Exploring Biology in the Laboratory, 3e, this Core Concepts edition features a streamlined set of clearly written activities with abbreviated coverage of the biodiversity of life. These exercises emphasize the unity of all living things and the evolutionary forces that have resulted in, and continue to act on, the diversity that we see around us today.

Enzymes in Detergency

Author: Jan H. van Ee,Onno Misset

Publisher: CRC Press

ISBN: 9780824799953

Category: Science

Page: 408

View: 9937


Offers an integrated overview of enzyme use in household detergents, from product development and manufacturing to safety and health-related issues. The text details the major types of enzymes, structure-function relationships, life cycle analyses, protein-engineering techniques, cleaning mechanisms, and past, present and future applications.

Enzyme Functionality

Design: Engineering, and Screening

Author: Allan Svendsen

Publisher: CRC Press

ISBN: 0824756916

Category: Medical

Page: 712

View: 6344


Enzyme Functionality serves as a conduit for trailblazing research in enzyme engineering-relating current understanding of sequence families, the new notion of enzyme structure classes, and modern methods in protein engineering, design, and directed evolution to accelerate the development of novel enzyme functionalities. This reference gathers the diverse perspectives of nearly 80 scientists from around the globe and surveys all leading rational and random approaches to the artificial evolution of enzymes. Citing more than 1500 notable works, it outlines assays for enzyme activity, stability, and specificity and a wide variety of site-directed, redesign, and evolutionary engineering methods.

Enzyme Kinetics: Catalysis and Control

A Reference of Theory and Best-Practice Methods

Author: Daniel L. Purich

Publisher: Elsevier

ISBN: 0123809258

Category: Science

Page: 920

View: 9213


Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. Historical analysis of kinetic principles including advanced enzyme science Provides both theoretical and practical measurements tools Coverage of single molecular kinetics Examination of force generation mechanisms Discussion of organic and inorganic enzyme reactions


Biochemistry, Biotechnology, Clinical Chemistry

Author: T Palmer,P L Bonner

Publisher: Elsevier

ISBN: 0857099922

Category: Technology & Engineering

Page: 432

View: 3352


In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student textbook has been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics, a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of biochemistry, and little of chemistry, is assumed. It is intended to provide an introduction to enzymology, and a balanced account of all the various theoretical and applied aspects of the subject which are likely to be included in a course. Provides an introduction to enzymology and a balanced account of the theoretical and applied aspects of the subject Discusses techniques such as membrane chromatography, aqueous phase partitioning and engineering recombinant proteins for purification Includes a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy

Principles of Enzyme Kinetics

Author: Athel Cornish-Bowden

Publisher: Elsevier

ISBN: 1483164675

Category: Science

Page: 220

View: 7524


Principles of Enzyme Kinetics discusses the principles of enzyme kinetics at an intermediate level. It is primarily written for first-year research students in enzyme kinetics. The book is composed of 10 chapters. Chapter 1 provides the basic principles of enzyme kinetics with a brief discussion of dimensional analysis. Subsequent chapters cover topics on the essential characteristics of steady-state kinetics, temperature dependence, methods for deriving steady-state rate equations, and control of enzyme activity. Integrated rate equations, and introductions to the study of fast reactions and the statistical aspects of enzyme kinetics are provided as well. Chemists and biochemists will find the book invaluable.

Enzyme Technology

Author: Ashok Pandey,Colin Webb,Carlos Ricardo Soccol,Christian Larroche

Publisher: Springer Science & Business Media

ISBN: 9780387292946

Category: Medical

Page: 742

View: 6678


This book is a unique resource for state-of-the-art research findings on biotechnological enzyme innovations. It provides a great deal of information on the potential of enzymes for commercial exploitation. The book includes photographs, figures, and tables.

Recombinant Enzymes - From Basic Science to Commercialization

Author: Azura Amid

Publisher: Springer

ISBN: 3319123971

Category: Science

Page: 188

View: 9219


This edited work presents studies that clarify the basics of producing recombinant enzymes that finally lead to commercialization. It enables researchers to see what is crucial to the commercialization process, from examining the cloning method, using analytical techniques such as calculating the total protein content and enzyme activity, through considering upstream and downstream processes, to the final product. Readers will discover the importance of the cloning method as it influences the upstream and downstream processes and determines the level of success of the recombinant enzyme commercialization processes. We see that the two main factors that are particularly sensitive during the cloning process are the vector and the host. A discussion of analytical techniques is presented followed by studies on important stages during the upstream processes including the process of optimizing the media to get results and high enzyme activity. Downstream processes such as the cell disruption technique, purification and formulation of the final product are then considered. The reader is introduced to software that helps streamline recombinant enzyme production from the upstream to downstream processes, to facilitate the process of up-scaling production. This work includes a case study as tool, to guide understanding of the commercialization process. The work is written for researchers in the field and is especially suited to those who are under pressure to embark on the tough process of commercialization.

Understanding Bacteria

Author: S. Srivastava

Publisher: Springer Science & Business Media

ISBN: 9781402016332

Category: Science

Page: 469

View: 1416


The discipline of microbiology that deals with an amazingly diverse group of simple organisms, such as viruses, archaea, bacteria, algae, fungi, and protozoa, is an exciting field of Science. Starting as a purely descriptive field, it has transformed into a truly experimental and interdisciplinary science inspiring a number of investigators to generate th a wealth of information on the entire gamut of microbiology. The later part of 20 century has been a golden era with molecular information coming in to unravel interesting insights ofthe microbial world. Ever since they were brought to light through a pair of ground glasses by the Dutchman, Antony van Leeuwenhoek, in later half of 17th century, they have been studied most extensively throughout the next three centuries, and are still revealing new facets of life and its functions. The interest in them, therefore, continues even in the 21 st century. Though they are simple, they provide a wealth of information on cell biology, physiology, biochemistry, ecology, and genetics and biotechnology. They, thus, constitute a model system to study a whole variety of subjects. All this provided the necessary impetus to write several valuable books on the subject of microbiology. While teaching a course of Microbial Genetics for the last 35 years at Delhi University, we strongly felt the need for authentic compiled data that could give exhaustive background information on each of the member groups that constitute the microbial world.

Stability and Stabilization of Enzymes

Proceedings of an International Symposium Held in Maastricht, The Netherlands, 22-25 November 1992

Author: W.J.J. van den Tweel,A. Harder,R.M. Buitelaar

Publisher: Elsevier

ISBN: 1483291332

Category: Science

Page: 535

View: 8827


These proceedings contain most of the oral presentations and posters of the international symposium on Stability and Stabilization of Enzymes held in Maastricht in November 1992. They provide a comprehensive overview of the state-of-the-art in this field. The possible applications of enzymes are enormous. Years of development have seen many enzymes brought onto the market, but they are still expensive to use. Therefore, their efficient application is a prerequisite for common usage. One of the main factors for this efficiency is the stability of the enzymes. The topics thus ranged from the extensive fundamental thermodynamic knowledge gathered in academic research to the practical applied knowledge built up in industry during the time that enzymes have been produced commercially. The subject Stability and Stabilization of Enzymes was discussed from various points of view, as was reflected in the themes of the symposium sessions. In the session on Fundamentals of Enzyme Stabilisation the thermodynamic background of the phenomenon was highlighted. In yet another session, the recently developed analytical tools to measure enzyme stability and stabilisation were discussed. Further sessions comprised the physical, chemical and biological ways to obtain enzyme stabilisation and finally, the industrial practice of enzyme stabilisation was treated by representatives of the world's most important enzyme producers. The book will be of interest to researchers in universities and industry in the fields of biochemistry, enzymology and biotechnology.

Structural and Mechanistic Enzymology

Bringing Together Experiments and Computing

Author: Christo Christov,Tatyana Karabencheva-Christova

Publisher: Academic Press

ISBN: 0123983126

Category: Science

Page: 459

View: 2881


Both strategies for investigation (computational and experimental) in structural and mechanistic Enzymology have developed to some extent independently. However, over the last few years a trend has emerged for strengthening their integration. This combination not only brings together computations and experiments focused on the same enzymatic problems, but also provides complementary insights into the investigated properties and has a powerful synergy effect. This thematic volume of Advances in Protein Chemistry and Structural Biology focuses on the recent success in structural and mechanistic enzymology and has its main emphasis on explaining the enzyme phenomena by using both the experimental and computational approaches. The selected contributions demonstrate how the application of a variety of experimental techniques and modeling methods helps further the understanding of enzyme dynamics, mechanism, inhibition, and drug design. Focuses on the recent success in structural and mechanistic enzymology. Has its main emphasis on explaining the enzyme phenomena by using both the experimental and computational approaches. Demonstrates how the application of a variety of experimental techniques and modeling methods helps further the understanding of enzyme dynamics, mechanism, inhibition, and drug design.

Mediale Medizin

Der wahre Ursprung von Krankheit und Heilung

Author: Anthony William

Publisher: Arkana

ISBN: 3641183901

Category: Body, Mind & Spirit

Page: 464

View: 942


Seit seiner Kindheit besitzt Anthony William eine einzigartige Gabe: Ein göttlicher Geist flüstert ihm äußerst präzise Informationen zum Gesundheitszustand seiner Mitmenschen ins Ohr. Diese medialen Botschaften zeigen nicht nur, an welcher Krankheit der Betroffene leidet, sondern auch, wie er wieder vollständig gesund wird – und sind dem heutigen Stand der Medizin oft um Lichtjahre voraus. Dieses Buch enthält erstmals das revolutionäre Heilwissen, das sich Anthony William in seiner langjährigen, erfolgreichen Praxis als medizinisches Medium erwarb. Es zeigt die wahren Ursachen von Krankheiten, die in der Medizinwissenschaft als chronisch oder unheilbar gelten, wie beispielsweise Rheumatoide Arthritis, Multiple Sklerose, Typ-2-Diabetes, hormonelle Erkrankungen, chronisches Müdigkeitssyndrom oder Borreliose. Neben der Ursache benennt William einfache, leicht gangbare Wege der Heilung: Detox, Superfoods, Ernährungstipps und eine reinigende 28-tägige Heilkur.