Protein Purification

Principles and Practice

Author: Robert K. Scopes

Publisher: Springer Science & Business Media

ISBN: 1475719574

Category: Science

Page: 329

View: 4804

DOWNLOAD NOW »

New textbooks at alllevels of chemistry appear with great regularity. Some fields like basic biochemistry, organic reaction mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a reallack ofup-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research which is advancing the field. lt is not often easy to persuade such individuals to settime aside to help spread the knowledge they have accumu lated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In some cases the availability of texts in active research areas should help stimulate the creation of new courses. NewYork CHARLES R. CANTOR Preface to the Second Edition The original plan for the first edition of this book was to title it Enzyme Purification: Princip/es and Practice.

Protein Purification

Principles and Practice

Author: Robert K. Scopes

Publisher: Springer Science & Business Media

ISBN: 1475723334

Category: Science

Page: 380

View: 1115

DOWNLOAD NOW »

New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic re action mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instruc tive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In so me cases the availability of texts in active research areas should help stimulate the creation of new courses.

Protein Purification

Principles and Practice

Author: Robert K. Scopes

Publisher: Springer Science & Business Media

ISBN: 9780387940724

Category: Science

Page: 380

View: 477

DOWNLOAD NOW »

New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic re action mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instruc tive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In so me cases the availability of texts in active research areas should help stimulate the creation of new courses.

Protein Purification

Principles, High Resolution Methods, and Applications

Author: Jan-Christer Janson

Publisher: John Wiley & Sons

ISBN: 1118002199

Category: Science

Page: 532

View: 5444

DOWNLOAD NOW »

The authoritative guide on protein purification—now completely updated and revised Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch. Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes: High-resolution reversed-phase liquid chromatography Electrophoresis in gels Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients Affinity ligands from chemical and biological combinatorial libraries Membrane separations Refolding of inclusion body proteins from E. coli Purification of PEGylated proteins High throughput screening techniques in protein purification The history of protein chromatography

Basic Methods in Protein Purification and Analysis

A Laboratory Manual

Author: Richard J. Simpson,Peter David Adams,Erica Golemis

Publisher: N.A

ISBN: 9780879698683

Category: Science

Page: 436

View: 7358

DOWNLOAD NOW »

A collection of convenient and easy to use, at the bench protocols for protein purification and further manipulations. Some of the methods describing protein purification are from Proteins and Proteomics and Purifying Proteins for Proteomics manuals, with additional information from Protein–Protein Interactions 2e (Standard Technologies).

Lectins and Glycobiology

Author: Hans-Joachim Gabius,Sigrun Gabius

Publisher: Springer Science & Business Media

ISBN: 3642779441

Category: Science

Page: 521

View: 7251

DOWNLOAD NOW »

The intriguing complexity, precision, and regulation of the wide range of biologi cal processes is determined by intricate mechanisms of molecular recognition. Their nature is under intense scrutiny. In addition to the well-appreciated interac tion of proteins either with amino acid or nucleotide sequences, the investigation of their interplay with carbohydrate elements of cellular glycoconjugates current ly exerts increasing attraction. In the group of carbohydrate-binding proteins, lec tins are distinguished from antibodies or ligand-affecting enzymes, according to the most recent definition. The thorough analysis of their structure and function is considered as a focus to collect a critical mass of information for delineating details of a further array of biochemical processes with pivotal physiological im pact. Following an already century-long history of scientific description, reflected by subjectively chosen highlights (see the Brief History of Lectin Research at page VI), the excitement in glycobiological research that prevails today can easily be ex plained by our growing awareness of the multifarious significance of a sugar-code system of biological information. This present notion unmistakably has an im pact on lines of research in diverse disciplines like cell and molecular biology, histochemistry, or clinical sciences. It also prompts inherent practical questions such as how to obtain lectins, or how to employ them as instruments in various assay systems with the best possible results. Thus, this book is devoted intentionally to cover the techniques in different research fields that deal with lectins.

Protein Purification Techniques

A Practical Approach

Author: Simon Roe

Publisher: OUP Oxford

ISBN: 9780199636730

Category: Technology & Engineering

Page: 278

View: 9310

DOWNLOAD NOW »

The protein purification Practical Approach books contain detailed practical information on the separation and purification of proteins. Protein Purification Techniques covers unit operations and analytical techniques in detail, while Protein Purification Applications has details of protein purification from a selected number of typical sources.

Fundamentals of Protein Structure and Function

Author: Engelbert Buxbaum

Publisher: Springer

ISBN: 331919920X

Category: Science

Page: 521

View: 815

DOWNLOAD NOW »

This book serves as an introduction to protein structure and function. Starting with their makeup from simple building blocks, called amino acids, the 3-dimensional structure of proteins is explained. This leads to a discussion how misfolding of proteins causes diseases like cancer, various encephalopathies, or diabetes. Enzymology and modern concepts of enzyme kinetics are then introduced, taking into account the physiological, pharmacological and medical significance of this often neglected topic. This is followed by thorough coverage of hæmoglobin and myoglobin, immunoproteins, motor proteins and movement, cell-cell interactions, molecular chaperones and chaperonins, transport of proteins to various cell compartments and solute transport across biological membranes. Proteins in the laboratory are also covered, including a detailed description of the purification and determination of proteins, as well as their characterisation for size and shape, structure and molecular interactions. The book emphasises the link between protein structure, physiological function and medical significance. This book can be used for graduate and advanced undergraduate classes covering protein structure and function and as an introductory text for researchers in protein biochemistry, molecular and cell biology, chemistry, biophysics, biomedicine and related courses. About the author: Dr. Buxbaum is a biochemist with interest in enzymology and protein science. He has been working on the biochemistry of membrane transport proteins for nearly thirty years and has taught courses in biochemistry and biomedicine at several universities.

Illustrated Guide to Home Chemistry Experiments

All Lab, No Lecture

Author: Robert Bruce Thompson

Publisher: "O'Reilly Media, Inc."

ISBN: 1449331424

Category: Science

Page: 432

View: 3091

DOWNLOAD NOW »

For students, DIY hobbyists, and science buffs, who can no longer get real chemistry sets, this one-of-a-kind guide explains how to set up and use a home chemistry lab, with step-by-step instructions for conducting experiments in basic chemistry -- not just to make pretty colors and stinky smells, but to learn how to do real lab work: Purify alcohol by distillation Produce hydrogen and oxygen gas by electrolysis Smelt metallic copper from copper ore you make yourself Analyze the makeup of seawater, bone, and other common substances Synthesize oil of wintergreen from aspirin and rayon fiber from paper Perform forensics tests for fingerprints, blood, drugs, and poisons and much more From the 1930s through the 1970s, chemistry sets were among the most popular Christmas gifts, selling in the millions. But two decades ago, real chemistry sets began to disappear as manufacturers and retailers became concerned about liability. ,em>The Illustrated Guide to Home Chemistry Experiments steps up to the plate with lessons on how to equip your home chemistry lab, master laboratory skills, and work safely in your lab. The bulk of this book consists of 17 hands-on chapters that include multiple laboratory sessions on the following topics: Separating Mixtures Solubility and Solutions Colligative Properties of Solutions Introduction to Chemical Reactions & Stoichiometry Reduction-Oxidation (Redox) Reactions Acid-Base Chemistry Chemical Kinetics Chemical Equilibrium and Le Chatelier's Principle Gas Chemistry Thermochemistry and Calorimetry Electrochemistry Photochemistry Colloids and Suspensions Qualitative Analysis Quantitative Analysis Synthesis of Useful Compounds Forensic Chemistry With plenty of full-color illustrations and photos, Illustrated Guide to Home Chemistry Experiments offers introductory level sessions suitable for a middle school or first-year high school chemistry laboratory course, and more advanced sessions suitable for students who intend to take the College Board Advanced Placement (AP) Chemistry exam. A student who completes all of the laboratories in this book will have done the equivalent of two full years of high school chemistry lab work or a first-year college general chemistry laboratory course. This hands-on introduction to real chemistry -- using real equipment, real chemicals, and real quantitative experiments -- is ideal for the many thousands of young people and adults who want to experience the magic of chemistry.

Protein Analysis and Purification

Benchtop Techniques

Author: I.M. Rosenberg

Publisher: Springer Science & Business Media

ISBN: 1475711085

Category: Mathematics

Page: 434

View: 9578

DOWNLOAD NOW »

This book is designed to be a practical progression of experimental techniques an investigator may follow when embarking on a biochemical project. The protocols may be performed in the order laid out or may be used inde pendently. The aim of the book is to assist a wide range of researchers. from the novice to the frustrated veteran, in the choice and design of experiments that are to be performed to provide answers to specific questions. The manual describes standard techniques that have been shown to work, as well as some newer ones that are beginning to prove important. By following the promi nently numbered steps. you can work your way through any protocol. whether it's a new technique or a task you've done before for which you need a quick review or updated methodology. This manual will assist the experimentalist in designing properly controlled experiments. There will be no advice for dealing with specific pieces of equip ment other than encouragement to read the manual, if you can find it. Through out all manipulations try to be objective. Be on the lookout for unexpected findings. You will learn the most from unexpected results. and they are often the beginning of the next project. It is never possible to record too much in your lab notebook. Do not get discouraged. Remember, things will not always run smoothly.

Protein Purification Protocols

Author: Paul Cutler

Publisher: Springer Science & Business Media

ISBN: 159259655X

Category: Science

Page: 484

View: 1956

DOWNLOAD NOW »

This new edition of Protein Purification Protocols completely updates the existing protocols to reflect recent advances and adds the enormous new array of proteomic techniques for protein isolation and analysis. These cutting-edge techniques include not only two-dimensional gel electrophoresis for analysis and characterization, but also analytical chromatography for multidimensional separations of proteins and peptides, and mass spectrometry for isolating proteins.

Bioorganic Chemistry

A Chemical Approach to Enzyme Action

Author: Hermann Dugas

Publisher: Springer Science & Business Media

ISBN: 1468403249

Category: Science

Page: 651

View: 4885

DOWNLOAD NOW »

Springer Advanced Texts In Chemistry New textbooks at all levels of chemistry appear with great regularity. Some fields like basic biochemistry, organic reaction mechanisms, and chemical thermody namics are well represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In some cases the availability of texts in active research areas should help stimulate the creation of new courses.

Biotechnology Proteins to PCR

A Course in Strategies and Lab Techniques

Author: David W. Burden,Donald B. Whitney

Publisher: Springer Science & Business Media

ISBN: 1461242789

Category: Science

Page: 317

View: 7921

DOWNLOAD NOW »

Preparative Chromatography Techniques

Applications in Natural Product Isolation

Author: K. Hostettmann,Andrew MARSTON,Maryse Hostettmann

Publisher: Springer Science & Business Media

ISBN: 3662036312

Category: Science

Page: 247

View: 7953

DOWNLOAD NOW »

Over the past few years, increasing attention has been paid to the search for bioactive compounds from natural sources. The success of plant-derived products such as paclitaxel (Taxol) in tumor therapy or artemisinin in the treatment of malaria has provided the impetus for the introduction of numerous research programmes, especially in Industry. A great deal of effort is being expended in the generation of novel lead molecules of vegetable, marine and microbial origin by the use of high throughput screening protocols. When interesting hits are found, it is essen tial to have methods available for the rapid isolation of target compounds. For this reason, both industry and academia need efficient preparative chromatographic separation techniques and experience in their application. Purified natural products are required for complete spectro scopic identification and full characterization of new compo unds, for biological testing and for the supply of pharmaceuti cals, standards, and starting materials for synthetic work. Obtaining pure products from an extract can be a very long, te dious and expensive undertaking, involving many steps. Sometimes only minute amounts of the desired compounds are at hand and these entities may be labile. Thus it is an advantage to have access to as many different methods as possible in order to aid the isolation process. Although a certain amount of trial and error may be involved, nowadays there is the possibility of devising suitable rapid separation schemes by a judicious choice of the different techniques available.

Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods

Author: T. Rabilloud

Publisher: Springer Science & Business Media

ISBN: 9783540657927

Category: Science

Page: 248

View: 2401

DOWNLOAD NOW »

With the end of the Human Genome Project in sight, the next important step is to determine the function of genes. Proteome Research is an important approach to this study and is the first book to comprehensively cover the application of two-dimensional electrophoresis, the central methodology in proteome research. The state-of-the-art is described in detail and the available detection methods are extensively covered. Sufficient detail is given to allow readers to apply these technologies to their own particular requirements.

Diagnostic Bacteriology Protocols

Author: Dr. Louise O'Connor

Publisher: Springer Science & Business Media

ISBN: 1597451436

Category: Science

Page: 225

View: 4355

DOWNLOAD NOW »

This fully updated edition includes the novel identification, detection, and typing technologies that are transforming the field of diagnostic testing. The authors, working at the forefront of diagnostic test development, highlight these new technologies and describe current and future molecular diagnostic tests and related nucleic acid extraction methods. The protocols range from advanced molecular detection, quantification, and typing systems, to protocols for diagnostic protein identification, serological testing, and cell culture-based assays. The emphasis is on nucleic acid-based diagnostics and alternative biochemically- and immunologically-based formats that can provide significant potential for multiparameter testing and automation.

Food Analysis

Author: S. Suzanne Nielsen

Publisher: Springer

ISBN: 3319457764

Category: Technology & Engineering

Page: 649

View: 5031

DOWNLOAD NOW »

This fifth edition provides information on techniques needed to analyze foods for chemical and physical properties. The book is ideal for undergraduate courses in food analysis and is also an invaluable reference to professionals in the food industry. General information chapters on regulations, labeling, sampling, and data handling provide background information for chapters on specific methods to determine chemical composition and characteristics, physical properties, and objectionable matter and constituents. Methods of analysis covered include information on the basic principles, advantages, limitations, and applications. Sections on spectroscopy and chromatography along with chapters on techniques such as immunoassays, thermal analysis, and microscopy from the perspective of their use in food analysis have been expanded. Instructors who adopt the textbook can contact the editor for access to a website with related teaching materials.

Methods in Protein Structure Analysis

Author: M. Zouhair Atassi,Ettore Appella

Publisher: Springer Science & Business Media

ISBN: 148991031X

Category: Science

Page: 534

View: 9331

DOWNLOAD NOW »

The MPSA international conference is held in a different country every two years. It is devoted to methods of determining protein structure with emphasis on chemistry and sequence analysis. Until the ninth conference, MPSA was an acronym for Methods in Protein Sequence Analysis. To give the conference more flexibility and breadth, the Scientific Advisory Committee of the lOth MPSA decided to change the name to Methods in Protein Structure Analysis; however, the emphasis remains on "methods" and on "chemistry. " In fact, this is the only major conference that is devoted to methods. The MPSA conference is truly international, a fact clearly reflected by the composi tion of its Scientific Advisory Committee. The Scientific Advisory Committee oversees the scientific direction of the MPSA and elects the chairman of the conference. Members of the committee are elected by active members, based on scientific standing and activity. The chairman, subject to approval of the Scientific Advisory Committee, appoints the Organizing Committee. It is this latter committee that puts the conference together. The lectures of the MPSA have traditionally been published in a special proceedings issue. This is different from, and more detailed than, the special MPSA issue of the Journal of Protein Chemistry in which only a brief description of the talks is given in short papers and abstracts. In the I Oth MPSA, about half the talks are by invited speakers and the remainder were selected from submitted short papers and abstracts.