Aqueous Two-phase Systems

Methods and Protocols

Author: Rajni Hatti-Kaul

Publisher: Springer Science & Business Media

ISBN: 1592590284

Category: Science

Page: 440

View: 9099


In Aqueous Two-Phase Systems: Methods and Protocols, Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications, all presented in set-by-step detail to ensure easy reproducibility and robust results. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins-including a glimpse of large-scale handling of two-phase separations. Techniques for in situ product recovery during biocatalytic processes and for polymer-polymer systems in organic solvents are also presented. Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.

Partitioning In Aqueous Two – Phase System

Theory, Methods, Uses, and Applications To Biotechnology

Author: Harry Walter

Publisher: Elsevier

ISBN: 0323142915

Category: Science

Page: 730

View: 1782


Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.

Aqueous Two-Phase Partitioning

Physical Chemistry and Bioanalytical Applications

Author: Boris Y. Zaslavsky

Publisher: CRC Press

ISBN: 9780824794613

Category: Science

Page: 656

View: 1468


Covers the fundamental principles of solute partitioning in aqueous two-phase systems, explains their important practical features, and furnishes methods of characterization. The information provided by the partition behaviour of a solute in an aqueous two-phase system is examined.

Aqueous Two-Phase Systems

Author: Harry Walter,Göte Johansson

Publisher: Elsevier

ISBN: 9780121821296

Category: Science

Page: 725

View: 7945


This volume of Methods in Enzymology represents the sole collection of separation methods based on aqueous two-phase systems. It includes procedures for the isolation of proteins, especially enzymes, nucleic acids, cell membranes, and organelles, as well as for the separation and study of cells. Key Features * Use of affinity partitioning for enzyme purification * Separation and study of cells * Isolation of plasma membranes * Large scale biotechnical use

Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products

Author: Marco Rito-Palomares,Jorge Benavides

Publisher: Springer

ISBN: 3319593099

Category: Technology & Engineering

Page: 212

View: 6281


This comprehensive and unique text presents a full overview of downstream processing useful for those new to the concept as well as professionals with experience in the area. The history and theoretical principles of Aqueous Two-Phase Systems (ATPS) are covered in depth. Information on ATPS characterization and application is included, and ATPS equilibria and system parameters that have significant effect on partition behavior are studied. Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products addresses specific applications of ATPS for the recovery and partial purification of high molecular weight compounds such as proteins, nucleic acids and polysaccharides, particulate bioproducts such as cells and organelles and low molecular weight compounds. Non-conventional strategies involving ATPS such as affinity systems, continuous liquid-liquid fractionation stages and the recovery from plant extracts are presented. Economic analysis of the application of ATPS in comparison to other fractionation techniques, particularly liquid chromatography, is considered, as are opportunity and current trends in the ATPS research area. Each chapter utilizes the contributors' experimental expertise in traditional and non-conventional ATPS strategies, as well as analysis of areas of opportunity and perspectives on the development and future applications of ATPS in both the lab and larger scale operations. The result is a thorough and singular overview of ATPS which has not been matched by any other text on the market.

Heme, Chlorophyll, and Bilins

Methods and Protocols

Author: Alison Smith,Michael Witty

Publisher: Springer Science & Business Media

ISBN: 1588291111

Category: Science

Page: 340

View: 2346


Since the discipline of handling and manipulating tetrapyrroles has been in practice for the past century, the material published in this field is seldom current and often does not include technical tips that can greatly influence the success of the provided methods for preparation and analysis. Handbooks have been published over the years that are now out-of-date and are theoretical rather practical in their orientation. In Heme, Chlorophyll, and Bilins: Methods and Protocols, scientists in the field provide detailed descriptions of basic protocols and technology for the study of chlorophyll, heme, and related molecules, including technical tips and suggestions to avoid common pitfalls. Simpler, more traditional methods, as well as the more modern and state-of-the-art procedures have been maintained, updated, and refined. This text is a current and comprehensive overview of chemical, biophysical, and molecular biological topics for accessible use by both experienced and novice laboratory researchers.

Handbook of Bioseparations

Author: Satinder Ahuja

Publisher: Elsevier

ISBN: 9780080507798

Category: Technology & Engineering

Page: 722

View: 7154


It is generally recognized that the commercial success of biotechnology products is highly dependent on the successful development and application of high-powered separation and purification methods. In this practical and authoritative handbook, the separation of proteins, nucleic acids, and oligonucleotides from biological matrices is covered from analytical to process scales. Also included in a chapter on the separation of monoclonal antibodies, which have found numerous uses as therapeutic and diagnostic agents. Analytical techniques include an interesting montage of chromatographic methods, capillary electrophoresis, isoelectric focusing, and mass spectrometry. Among separation and purification methods, liquid-liquid distribution, displacement chromatography, expanded bed adsorption, membrane chromatography, and simulated moving bed chromatography are covered at length. Regulatory and economic considerations are addressed, as are plant and process equipment and engineering process control. A chapter on future developments highlights the application of DNA chip arrays as well as evolving methodologies for a large number of drugs that are under development for treatment of cancer, AIDS, rheumatoid arthritis, and Alzheimer's disease. Handbook of Bioseparations serves as an essential reference and guidebook for separation scientists working in the pharmaceutical and biotechnology industries, academia, and government laboratories. Key Features * Covers bioseparations of proteins, nucleic acids, and monoclonal antibodies * Encompasses both analytical and process-scale methods * Elucidates the importance of engineering process control * Details selection of plant and process equipment * Addresses economic considerations * Discusses future developments

Separations Using Aqueous Phase Systems

Applications in Cell Biology and Biotechnology

Author: D. Fisher

Publisher: Springer Science & Business Media

ISBN: 1468456679

Category: Science

Page: 504

View: 9547


The use of aqueous two-phase systems for the partitioning of macromolecules, organelles and cells was originally developed by Per-Ake Albertsson in Sweden in the mid-fifties [1-3]. These systems were initially applied to separations of plant organelles and viruses but their use has now extended into most areas of cell biology and biochemistry [4,5]. Since 1979 biennial International Conferences on Partitioning in Aqueous Two-Phase Systems have been held in Los Angeles (1979), Sheffield (1981), Vancouver (1983) and Lund (1985). The 5th conference was held in Oxford from 23-28 August 1987 and was entitled "Advances in Separations Using Aqueous Phase Systems in Cell Biology and Biotechnology". It is the formal presentations from this meeting which comprise this volume. In contrast to earlier books on phase partitioning [4,5] this volume contains, for the first time, worldwide contributions from over sixty partitioners from a variety of scientific disciplines, thereby providing a detailed overview of the widespread application and potential of bioseparations using phase partitioning. Disciplines include Biophysics, Biochemistry, Cell Biology, Microbiology, Biotechnology and Process Engineering, in both academic and commercial establishments. These biennial conferences allow advances in these diverse partitioning fields to be reviewed and compared; they also provide an opportunity for those considering using phase partitioning to obtain information, advice and contacts. Attendance has grown steadily over the years and 140 scientists came to Oxford. The conference consisted of ten symposia on areas of application of partitioning which have been organised as specific chapters in this volume.

Poly(Ethylene Glycol) Chemistry

Biotechnical and Biomedical Applications

Author: J. Milton Harris

Publisher: Springer Science & Business Media

ISBN: 1489907033

Category: Medical

Page: 385

View: 5515


The idea for this book came from discussions among participants in a symposium on biotechnical applications at the "Pacifichem 89" meeting in Honolulu. It was the majority opinion of this group that a volume dedicated to biotechnical and biomedical applications of PEG chemistry would enhance research and development in this area. Though the book was conceived at the Honolulu meeting, it is not a proceedings of this symposium. Several groups who did not participate in this meeting are repre sented in the book, and the book incorporates much work done after the meeting. The book does not include contributions in all related areas to which PEG chemistry has been applied. Several invited researchers declined to parti.:ipate, and there is not enough space in this single volume to properly cover all submissions. Chapter I-an overview of the topic-discusses in brief applications not given detailed coverage in specifically devoted chapters. The following topics are covered: introduction to and fundamental properties of PEG and derivatives in Chapters 1-3; separations using aqueous polymer two-phase partitioning in Chapters 4-6; PEG-proteins as catalysts in biotechnical applications in Chapters 7 and 8; biomedical applications of PEG-proteins in Chapters 9-13; PEG modified surfaces for a variety of biomedical and biotechnical applications in Chapters 14-20; and synthesis of new PEG derivatives in Chapters 21 and 22.

Protein Structure Analysis

Preparation, Characterization, and Microsequencing

Author: Roza Maria Kamp,Theodora Choli-Papadopoulou,Brigitte Wittmann-Liebold

Publisher: Springer Science & Business Media

ISBN: 9783540615002

Category: Science

Page: 316

View: 2642


"Protein Structure Analysis - Preparation and Characterization" is a compilation of practical approaches to the structural analysis of proteins and peptides. Here, about 20 authors describe and comment on techniques for sensitive protein purification and analysis. These methods are used worldwide in biochemical and biotechnical research currently being carried out in pharmaceu tical and biomedical laboratories or protein sequencing facilities. The chapters have been written by scientists with extensive ex perience in these fields, and the practical parts are well documen ted so that the reader should be able to easily reproduce the described techniques. The methods compiled in this book were demonstrated in student courses and in the EMBO Practical Course on "Microsequence Analysis of Proteins" held in Berlin September 10-15, 1995. The topics also derived from a FEBS Workshop, held in Halkidiki, Thessaloniki, Greece, in April, 1995. Most of the authors participated in these courses as lecturers and tutors and made these courses extremely lively and successful. Since polypeptides greatly vary depending on their specific structure and function, strategies for their structural analysis must for the most part be adapted to each individual protein. Therefore, advantages and limitations of the experimen tal approaches are discussed here critically, so that the reader becomes familiar with problems that might be encountered.

Molecular Biology and Biotechnology

Author: John M. Walker,Ralph Rapley

Publisher: Royal Society of Chemistry

ISBN: 0854041257

Category: Science

Page: 604

View: 1708


This popular textbook has been revised and updated to provide a comprehensive overview and to reflect the latest developments in this rapidly developing area. Continuing with the broad base style of both current molecular and traditional biotechnology, chapters have been updated to reflect current interest and include new areas such as stem cell technology and important areas in drug discovery such as IP and patents. By presenting information in an easily assimilated form, this book makes an ideal undergraduate text for students of biology and chemistry, as well as to postgraduates.

Aqueous Biphasic Separations

Biomolecules to Metal Ions

Author: Robin D. Rogers,M.A. Eiteman

Publisher: Springer Science & Business Media

ISBN: 1461519535

Category: Science

Page: 191

View: 9209


Proceedings of an American Chemical Society Symposium held in San Diego, California, March 13-14, 1994